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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
22/07/2018 |
Actualizado : |
22/07/2018 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
FERNÁNDEZ, S.; FRAGA, M.; SILVEYRA, E.; TROMBERT, A.N.; RABAZA, A.; PLA, M. |
Afiliación : |
S. FERNÁNDEZ, MEC/ IIBCE (Instituto de Investigaciones de Ciencias Biológicas "Clemente Estable"); MARTIN FRAGA COTELO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; E. SILVEYRA, MEC/ IIBCE (Instituto de Investigaciones de Ciencias Biológicas "Clemente Estable"); A.N. TROMBERT, Universidad Mayor, Santiago (Chile); A. RABAZA, MEC/ IIBCE (Instituto de Investigaciones de Ciencias Biológicas "Clemente Estable"); MARCELO PLA TEJERA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Probiotic properties of native Lactobacillus spp. strains for dairy calves. |
Complemento del título : |
Research article. |
Fecha de publicación : |
2018 |
Fuente / Imprenta : |
Beneficial Microbes, April 2018, vol.9, no.4, p. 613 - 624. |
ISSN : |
1876-2883 |
DOI : |
10.3920/BM2017.0131 |
Idioma : |
Inglés |
Notas : |
Artilce history: Received: 12 September 2017 / Accepted: 22 December 2017 / Published Online: April 10, 2018. |
Contenido : |
ABSTRACT.
The use of native microorganisms with probiotic capacity is an alternative tool for the treatment and prevention of several diseases that affect animals, such as neonatal calf diarrhoea. The selection of probiotic strains within a collection is based on different in vitro and in vivo assays, which predict their potential. The aim of this study was to characterise a group of native Lactobacillus spp. strains isolated from faeces of healthy calves using an in vitro approach and to assess their ability to colonise the gastrointestinal tract (GIT) of calves. Native Lactobacillus spp. strains were evaluated on their capacity to survive low pH conditions and bile salts presence, biofilm formation and adhesion to both mucus and Caco-2 cells. Based on the in vitro characterisation, four strains (Lactobacillus johnsonii TP1.1, Lactobacillus reuteri TP1.3B, L. johnsonii TP1.6 and Lactobacillus amylovorus TP8.7) were selected to evaluate their capacity to colonise and persist in the GIT of calves. The assessment of enteric persistence involved an in vivo assay with oral administration of probiotics and quantification in faeces of the administered bacterial species with real-time quantitative PCR (qPCR). The study was conducted using 15 calves (1-month-old) which were divided into five groups of three animals, four of which were treated with four different selected strains and one was the control group. Strains TP1.3B and TP1.6 managed to persist in treated animals until ten days after the end of the administration period, indicating that they could be promising candidates for the design of probiotics for calves.
© 2018 Wageningen Academic Publishers. MenosABSTRACT.
The use of native microorganisms with probiotic capacity is an alternative tool for the treatment and prevention of several diseases that affect animals, such as neonatal calf diarrhoea. The selection of probiotic strains within a collection is based on different in vitro and in vivo assays, which predict their potential. The aim of this study was to characterise a group of native Lactobacillus spp. strains isolated from faeces of healthy calves using an in vitro approach and to assess their ability to colonise the gastrointestinal tract (GIT) of calves. Native Lactobacillus spp. strains were evaluated on their capacity to survive low pH conditions and bile salts presence, biofilm formation and adhesion to both mucus and Caco-2 cells. Based on the in vitro characterisation, four strains (Lactobacillus johnsonii TP1.1, Lactobacillus reuteri TP1.3B, L. johnsonii TP1.6 and Lactobacillus amylovorus TP8.7) were selected to evaluate their capacity to colonise and persist in the GIT of calves. The assessment of enteric persistence involved an in vivo assay with oral administration of probiotics and quantification in faeces of the administered bacterial species with real-time quantitative PCR (qPCR). The study was conducted using 15 calves (1-month-old) which were divided into five groups of three animals, four of which were treated with four different selected strains and one was the control group. Strains TP1.3B and TP1.6 managed to persist in treated animals until ten d... Presentar Todo |
Palabras claves : |
GASTROINTESTINAL TRACT; LACTOBACILLUS SPP; NATIVE; NEONATAL CALF DIARRHOEA; PROBIOTIC POTENTIAL. |
Thesagro : |
GANADO DE LECHE. |
Asunto categoría : |
-- |
Marc : |
LEADER 02595naa a2200289 a 4500 001 1058830 005 2018-07-22 008 2018 bl uuuu u00u1 u #d 022 $a1876-2883 024 7 $a10.3920/BM2017.0131$2DOI 100 1 $aFERNÁNDEZ, S. 245 $aProbiotic properties of native Lactobacillus spp. strains for dairy calves.$h[electronic resource] 260 $c2018 500 $aArtilce history: Received: 12 September 2017 / Accepted: 22 December 2017 / Published Online: April 10, 2018. 520 $aABSTRACT. The use of native microorganisms with probiotic capacity is an alternative tool for the treatment and prevention of several diseases that affect animals, such as neonatal calf diarrhoea. The selection of probiotic strains within a collection is based on different in vitro and in vivo assays, which predict their potential. The aim of this study was to characterise a group of native Lactobacillus spp. strains isolated from faeces of healthy calves using an in vitro approach and to assess their ability to colonise the gastrointestinal tract (GIT) of calves. Native Lactobacillus spp. strains were evaluated on their capacity to survive low pH conditions and bile salts presence, biofilm formation and adhesion to both mucus and Caco-2 cells. Based on the in vitro characterisation, four strains (Lactobacillus johnsonii TP1.1, Lactobacillus reuteri TP1.3B, L. johnsonii TP1.6 and Lactobacillus amylovorus TP8.7) were selected to evaluate their capacity to colonise and persist in the GIT of calves. The assessment of enteric persistence involved an in vivo assay with oral administration of probiotics and quantification in faeces of the administered bacterial species with real-time quantitative PCR (qPCR). The study was conducted using 15 calves (1-month-old) which were divided into five groups of three animals, four of which were treated with four different selected strains and one was the control group. Strains TP1.3B and TP1.6 managed to persist in treated animals until ten days after the end of the administration period, indicating that they could be promising candidates for the design of probiotics for calves. © 2018 Wageningen Academic Publishers. 650 $aGANADO DE LECHE 653 $aGASTROINTESTINAL TRACT 653 $aLACTOBACILLUS SPP 653 $aNATIVE 653 $aNEONATAL CALF DIARRHOEA 653 $aPROBIOTIC POTENTIAL 700 1 $aFRAGA, M. 700 1 $aSILVEYRA, E. 700 1 $aTROMBERT, A.N. 700 1 $aRABAZA, A. 700 1 $aPLA, M. 773 $tBeneficial Microbes, April 2018, vol.9, no.4, p. 613 - 624.
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INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA Tacuarembó. |
Fecha actual : |
21/02/2014 |
Actualizado : |
27/08/2019 |
Tipo de producción científica : |
Documentos |
Autor : |
INIA TACUAREMBÓ; RISSO, D.; BERRETTA, E.J.; OLMOS, F.; BEMHAJA, M. |
Afiliación : |
ESTACIÓN EXPERIMENTAL DEL NORTE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; DIEGO FERNANDO RISSO RIET, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ELBIO JOAQUIN BERRETTA CARVALLO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FERNANDO ELCEAR OLMOS LOPEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIA DE LURDES BEMHAJA SARAIVA FERREIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Pasturas en suelos arenosos. |
Complemento del título : |
LA MAGNOLIA, DÍA DE CAMPO, 16 DE SETIEMBRE, TACUAREMBÓ, URUGUAY, 1993. |
Fecha de publicación : |
1993 |
Fuente / Imprenta : |
Tacuarembó (Uruguay): INIA, 1993. |
Páginas : |
21 p. |
Idioma : |
Español |
Contenido : |
Introducción: Diego Risso; Variación estacional en las condiciones del tiempo: La Magnolia, junio 1992 a junio 1993: María Bemhaja, Carlos Picos; Estudios para la implementación de sistemas agro-silvo-pastoriles: Fernando Olmos, Gustavo Balmelli, Enrique Pérez Gomar; Bromus Auleticus: María Bemhaja; Estudios sobre el género Ornithopus: Fernando Olmos; Evaluación de triticale consociado con leguminosas: María Bemhaja, Ángel Zarza. Leguminosas sembradas en consociación con triticale: María Bemhaja; Raigrás: Fertilización nitrogenada: María Bemhaja, Enrique Pérez Gomar; Caracterización de suelos arenosos en La Magnolia: con y sin historia agrícola: Enrique Pérez Gomar, María Bemhaja; Recuperación de suelo con historia agrícola y hortícola: María Bemhaja, Gustavo Pereira, Enrique Pérez Gomar, Ángel Zarza; Pasto elefante: Gramínea perenne estival en suelos arenosos erosionados: María Bemhaja, Guillermo Pigurina, G. Scaglia. |
Palabras claves : |
CAMPO NATURAL; PASTO ELEFANTE; PASTURE; PRODUCCIÓN FORRAJERA. |
Thesagro : |
SUELO ARENOSO. |
Asunto categoría : |
A50 Investigación agraria |
Marc : |
LEADER 01513nam a2200229 a 4500 001 1023691 005 2019-08-27 008 1993 bl uuuu u0uu1 u #d 100 1 $aINIA TACUAREMBÓ 245 $aPasturas en suelos arenosos. 260 $aTacuarembó (Uruguay): INIA$c1993 300 $a21 p. 520 $aIntroducción: Diego Risso; Variación estacional en las condiciones del tiempo: La Magnolia, junio 1992 a junio 1993: María Bemhaja, Carlos Picos; Estudios para la implementación de sistemas agro-silvo-pastoriles: Fernando Olmos, Gustavo Balmelli, Enrique Pérez Gomar; Bromus Auleticus: María Bemhaja; Estudios sobre el género Ornithopus: Fernando Olmos; Evaluación de triticale consociado con leguminosas: María Bemhaja, Ángel Zarza. Leguminosas sembradas en consociación con triticale: María Bemhaja; Raigrás: Fertilización nitrogenada: María Bemhaja, Enrique Pérez Gomar; Caracterización de suelos arenosos en La Magnolia: con y sin historia agrícola: Enrique Pérez Gomar, María Bemhaja; Recuperación de suelo con historia agrícola y hortícola: María Bemhaja, Gustavo Pereira, Enrique Pérez Gomar, Ángel Zarza; Pasto elefante: Gramínea perenne estival en suelos arenosos erosionados: María Bemhaja, Guillermo Pigurina, G. Scaglia. 650 $aSUELO ARENOSO 653 $aCAMPO NATURAL 653 $aPASTO ELEFANTE 653 $aPASTURE 653 $aPRODUCCIÓN FORRAJERA 700 1 $aRISSO, D. 700 1 $aBERRETTA, E.J. 700 1 $aOLMOS, F. 700 1 $aBEMHAJA, M.
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